After breakfast, we make an expedition to Sierra Negra Volcano, considered the oldest of the volcanoes on Isabela, the crater of Sierra Negra has a 10 km diameter and it’s the second largest in the world; its last eruption occurred in 2005. The Sulfur, iron and the cooled magma make up a colorful landscape. Our adventure begins with a 45 minutes ride on a bus to the highlands of Isabela, we arrive at “Paradero Sierra Negra”, from here we walk through slopes of the Volcano. The trail conducts us to Volcan Chico (on the northeast of Sierra Negra Volcano), a fissure composed of scum and lava. This fissure had its last eruption on 1979 and the volcanic activity lasted for a week. From here we have an amazing view of the north of the island and if the day is clear we have a majestic view of the Perry Isthmus, a surrounding island. This expedition allows us to step through volcanic features and over lava flows formed in very recent years. We also see the formations of petrified lava of many different colors and textures and fumaroles scattered throughout this area. Return to the Yacht for lunch. In the afternoon we visit Humedales, where we can see series of lagoons, mangrove species, flamingos and numerous migratory birds. After that, we visit Breeding Center “Arnaldo Tupiza”, where we can observe tortoises of different ages and sizes (In total there are 330 between juvenile and adult tortoises) and where we can learn about the reproduction of the giant tortoises, in the Breeding Center we can also see different native plants. Return to the Yacht for a delicious dinner and our nightly orientation.